Role of Iron in Salmonelaa Typhimurium growth
Abstract: Salmonella Typhimurium is an intestinal foodborne pathogen infecting both humans and animals. Infection begins with the ingestion of contaminated food or water and results in fever, cramping, vomiting, and diarrhea as the pathogen reaches the intestinal epithelium and triggers inflammation. Salmonella can survive the extreme conditions of the intestine, such as the low pH of the stomach, antimicrobial action of bile salts in the small intestine, as well as low nutrient content and anaerobic environment in the large intestine. In some cases, S. Typhimurium can form biofilms which may lead to chronic, reoccurring infections. To survive, S. Typhimurium needs carbon sources in the form of carbohydrates or short chain fatty acids to survive. Additionally, the pathogen requires trace elements, specifically Iron(III). These nutrients are often in limited supply in the intestines, requiring S. Typhimurium to compete with the host or the commensal bacterial. To better understand the role of iron in growth and biofilm formation, wild-type Salmonella and a S. Typhimurium strain lacking the Salmonella ABC-transporter iroC (ΔiroC) were assayed for growth and biofilm assays. If S. Typhimurium relies on Iron(III) for growth and biofilm formation, we expect to see alterations in growth rate and ability to form biofilms between media formulations and between S. Typhimurium cell lines.
Jacob Newsome*, Richard Laughlin, and Victoria Posada
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