Green Biosynthesis of Silver Nanoparticles That Are Effective Against Common Foodborne Pathogens
Abstract: The synthesis of noble metal nanoparticles using plant extracts has long intrigued the research world. Recent studies have shown that nanoparticles derived from noble metals such as silver have broad potential applications in the agriculture and biomedical fields. Conventional methods in nanoparticle synthesis require the use of dangerous, toxic chemicals and large amount of heat (energy) resulting in the formation of hazardous byproducts. In this study, Phyla dulcis plant extract-mediated synthesis of silver nanoparticles (AgNPs) was investigated. The fresh plant material was used to prepare the extract and reacted with AgNO3 to form AgNPs. Formation of AgNPs was confirmed using UV-Vis spectroscopy by observing peaks in the range of 425 nm – 450 nm due to surface plasmon resonance (SPR). The visible perception of color change from golden yellow to dark brown also indicated the formation of AgNPs. The zeta potential measurement resulted in an average value of -22.0 mV indicating that the AgNPs are stable. Also, the size distribution analysis conducted using Zetasizer Nanoseries showed that the particles are within nano scale. X-ray photoelectron spectroscopy (XPS) confirmed the formation of metallic silver nanoparticles by reduction of silver ions used in the synthesis. The composition of nanoparticles was further confirmed by Scanning Electron Microscopy/Energy Dispersive X-ray Spectroscopy (SEM-EDS) techniques. Antimicrobial studies revealed the ability of the nanoparticles to inhibit the growth of Salmonella typhimurium, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes; which are known to be common food borne pathogens.
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